Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay

Kazumasa Hirata; Mari Kobayashi; Kazuhisa Miyamoto; Toru Hoshi; Mitsuo Okazaki; Yoshiharu Miura

doi:10.1055/s-2006-961999

Planta Medica, Table of Contents

Planta Med 1989; 55(3): 262-264
DOI: 10.1055/s-2006-961999

Papers

Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay

Kazumasa Hirata¹ , Mari Kobayashi¹ , Kazuhisa Miyamoto¹ , Toru Hoshi² , Mitsuo Okazaki³ , Yoshiharu Miura¹

¹Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, 565, Japan
²Research and Development Division, Asahi Glass Co., Ltd., Hazawa-cho, Kanagawa-ku, Yokohama, 221, Japan
³Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1, Tokida, Ueda, 386, Japan

Abstract

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Abstract

A radioimmunoassay has been developed for the quantitative determination of small amounts of vinblastine in tissue cultures of Catharanthus roseus (L.) G. Don. Antibody was obtained by the immunization of rabbits against a conjugate of vinblastine with bovine serum albumin. The antibody had a high affinity (Ka = 1.2 × 10⁹l/mol) and specificity for vinblastine. The usable range of standard curve for assay was 0.5-10 ng/ml. Crude alkaloid extracts of tissue cultures could be assayed and many samples could be processed in one time. It was found that the vinblastine contents of multiple shoot cultures were lower than that of intact plants but much higher than that of callus cultures.

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